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KMID : 0624620170500080423
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BMB Reports
2017 Volume.50 No. 8 p.423 ~ p.428
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SRSF2 directly inhibits intron splicing to suppresses cassette exon inclusion
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Moon Hee-Gyum
Cho Sung-Hee
Loh Tiing Jen
Jang Ha-Na
Liu Yongchao
Choi Nam-Jeong
Oh Ja-Gyeong
Ha Ji-Yeon
Zhou Jianhua
Cho Sung-Chan
Kim Dong-Eun
Ye Michael B.
Zheng Xuexiu
Shen Haihong
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Abstract
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SRSF2, a Serine-Arginine rich (SR) protein, is a splicing activator that mediates exon inclusion and exclusion events equally well. Here we show SRSF2 directly suppresses intron splicing to suppress cassette exon inclusion in SMN pre-mRNA. Through a serial mutagenesis, we demonstrate that a 10 nt RNA sequence surrounding the branch-point (BP), is important for SRSF2-mediated inhibition of cassette exon inclusion through directly interacting with SRSF2. We conclude that SRSF2 inhibits intron splicing to promote exon exclusion.
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KEYWORD
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Exon exclusion, Intron retention, Pre-mRNA splicing, SRSF2
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